Genetic and Functional Analysis of the Role of Clonal Tumor Infiltrating Leukocytes in Breast Cancer Pathogenesis and Therapy

E Comen… - 2018 - apps.dtic.mil
bone marrow with BRC3 cells and their associated stroma. In … and sample preparation for
downstream analysis including … performs mouse irradiation, tail vein injections, bone marrow

Genetic and Functional Analysis of the Role of Clonal Tumor-Infiltrating Leukocytes in Breast Cancer Pathogenesis and Therapy

JS Reis-Filho… - 2018 - apps.dtic.mil
… or wildtype bone marrow with BRC3, Py230 or Py8119 cells and their associated stroma. In
… , DNA extraction and sample preparation for downstream analysis including flow cytometry …

[HTML][HTML] THBS1-producing tumor-infiltrating monocyte-like cells contribute to immunosuppression and metastasis in colorectal cancer

M Omatsu, Y Nakanishi, K Iwane, N Aoyama… - Nature …, 2023 - nature.com
… This study highlights the critical importance of bone marrow (… MTO-inoculated mice, like that
in human CRC, and its stroma-… mice into WT mice and generated GFP-BM chimeric mice for …

Bone marrow-derived cells are recruited by the melanoma tumor with endothelial cells contributing to tumor vasculature

R Bonfim-Silva, LEB Souza, FUF Melo… - Clinical and …, 2017 - Springer
… µm and put in slides prepared with polylysine (SIGMA… cells grafting (CD31+) into bone
marrow of chimeric mice and detection and quantification of CD31+/GFP+ cells in the tumor stroma

[HTML][HTML] Cell intrinsic deregulated ss-catenin signaling promotes expansion of bone marrow derived connective tissue type mast cells, systemic inflammation, and …

A Saadalla, MM Lima, F Tsai, A Osman… - Frontiers in …, 2019 - frontiersin.org
… performed Western blot analysis of cell extracts prepared from heterozygous 5CreCAT MCs,
stromal localization identifies CTMCs (2–4). While we cannot rule out that tumor infiltrating

[HTML][HTML] Hedgehog-induced PD-L1 on tumor-associated macrophages is critical for suppression of tumor-infiltrating CD8+ T cell function

AJ Petty, R Dai, R Lapalombella, RA Baiocchi… - JCI insight, 2021 - ncbi.nlm.nih.gov
… In this study, we first showed that tumor stroma-derived PD-… PCT (qRT-PCR) using bone
marrow–derived macrophages (BMDMs) … To prepare macrophages, mice were sacrificed and …

Inhibitory functions of PD-L1 and PD-L2 in the regulation of anti-tumor immunity in murine tumor microenvironment

D Umezu, N Okada, Y Sakoda, K Adachi… - Cancer Immunology …, 2019 - Springer
… Experiments using bone marrow (BM) chimeric mice, … Tumor-infiltrating immune cells were
separated from tumor … were prepared and statistical differences were analyzed using the log-…

TNFα and radioresistant stromal cells are essential for therapeutic efficacy of cyclic dinucleotide STING agonists in nonimmunogenic tumors

BJ Francica, A Ghasemzadeh, AL Desbien… - Cancer immunology …, 2018 - AACR
Through the use of bone marrow chimeras, we provide … Single-cell suspensions were
prepared by dissociation with … reduction in the proportion of tumor-infiltrating lymphocytes and …

[HTML][HTML] … -Specific CD8+ T Cells from the Bone Marrow Resist Exhaustion and Exhibit Increased Persistence in Tumor-Bearing Hosts as Compared to Tumor Infiltrating …

EM Zawidzka, L Biavati, A Thomas, C Zanettini… - bioRxiv, 2023 - ncbi.nlm.nih.gov
… Strategies employing adoptive cell therapy (ACT) have seen the establishment of chimeric
tumor injection, bone marrow was harvested from 5 mice, processed, and prepared for sorting…

Exogenous lipid uptake induces metabolic and functional reprogramming of tumor-associated myeloid-derived suppressor cells

AA Al-Khami, L Zheng, L Del Valle, F Hossain… - …, 2017 - Taylor & Francis
… the mechanisms by which tumor-infiltrating MDSC increase the … that found in TES or ascites
preparations, which involve dilution in … Bone marrow (BM) chimeras were set up as described …